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For Sale Magnetite Separation

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For Sale Magnetite Separation

Pellet protein G magnetic beads by placing the tubes in a magnetic separation rack and wait 1 to 2 min for solution to clear. Carefully transfer eluted chromatin supernatant to a new tube. To all tubes, including the 2% input sample from Step 1, reverse cross-links by adding 6 l 5M NaCl and 2 l Proteinase K #10012, and incubate 2 h at 65 C.

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